Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Blood
Cell type
OPM-2
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

source_name
OPM2
cell line
OPM2
cell type
multiple myeloma cell line
treatment
DMSO
chip antibody
MYC (Cell Signaling Technology, 18583)

Sequenced DNA Library

library_name
GSM6508886
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
OPM2 cells were crosslinked, lysed and sonicated. For ChIP with reference exogenous genome, spike-in chromatin (Active Motif, 53083) was then added. The protein-bound DNA fragments were then immunoprecipitated with specific antibodies. After reverse-crosslinking, DNA was purified. Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics) according to the manufacturer's instructions. Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
57546597
Reads aligned (%)
97.6
Duplicates removed (%)
5.8
Number of peaks
6878 (qval < 1E-05)

hg19

Number of total reads
57546597
Reads aligned (%)
96.8
Duplicates removed (%)
6.1
Number of peaks
5800 (qval < 1E-05)

Base call quality data from DBCLS SRA